I am unable to open my files once they are downloaded
What freeware is available to view my files?
A compressed folder of your Sanger sequencing ab1 files is made available in a folder named by date of when the data was uploaded. Typically, samples submitted by 10 am will be processed that day and results will be uploaded the next business day. Samples are analyzed with the KB basecaller program. For additonal information on Sanger services visit the Sanger Sequencing page.
Accurate sequencing data can only be obtained from the electropherogram, so it is very important to look at it and not just the sequence. Below are several programs available to visualize Sanger sequencing files. Note, some are free and some are available for purchase:
- Sequence Scanner v2.0
- Chromas (PC)
- Lasergene (PC/Mac, available through the RTSF for a small fee. Please email Ashley Shillinger, firstname.lastname@example.org for more information.)
- 4Peaks (Mac)
- CodonCode Aligner (Mac)
A compressed folder of your Real-Time qPCR EDS file is made available in a folder named by date of when the data was uploaded. Typically. samples submitted by 4pm will be set up to run that day and results will be uploaded the next business day.
Applied Biosystems QuantStudio™ 6 & 7 Flex Real-Time PCR System software can be downloaded to analyze your data:GeneScan/Fragment Analysis
GeneScan and Fragment Analysis data is provided in .fsa file format and is made available in a folder named by date of when the data was uploaded. The files can be viewed using the GeneScan software which is available on a computer in the Genomics Core foyer or via Peak Scanner. Peak Scanner is a freeware that can be downloaded from the Thermo Fisher/Invitrogen/Applied Biosystems website:
A compressed folder of your RCC files is made available in a folder named by date of when the data was uploaded. NanoString's nSolver software is needed to analyze the data and can be downloaded from NanoString's website:
A compressed folder of your SmartChip data is made available in a folder named by date of when the data was uploaded.Cell ID
Cell ID data is provided in an excel table that has been exportedc from the Genotyper software, The excel file shows the sample genotypes for each of the markers. There will also be a summary table tab showing the results for each sample.